Selected Publications

Singleton MR, Sawaya MR, Ellenberger T and Wigley DB. Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides. Cell 2000; 101: 589-600. PubMed

Singleton MR, Scaife S and Wigley DB. Structural analysis of DNA replication fork reversal by RecG. Cell 2001; 107: 79-89. PubMed

Singleton MR, Wentzell LM, Liu YL, West SC, Wigley DB. Structure of the single-strand annealing domain of human RAD52 protein. Proc Natl Acad Sci USA 2002; 99: 13492-13497. PubMed PDF (UKPMC)

Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB. Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature 2004; 432: 187-193. PubMed

Seybert A, Wigley DB. Distinct roles for ATP binding and hydrolysis at individual subunits of an archaeal clamp loader. EMBO J 2004; 23: 1360-1371. PubMed PDF (UKPMC)

Primary Research Publications

2009

Lucarelli D, Wang YA, Galkin VE, Yu X, Wigley DB, Egelman EH. The RecB nuclease domain binds to RecA-DNA filaments: implications for filament loading. J Mol Biol 2009 Aug 14;391(2):269-74.

Saikrishnan K, Powell B, Cook NJ, Webb MR, Wigley DB. Mechanistic basis of 5'-3' translocation in SF1B helicases. Cell 2009 May 29;137(5):849-59.

Wigley DB. ORC proteins: marking the start. Curr Opin Struct Biol 2009 Feb;19(1):72-8.

2008

Saikrishnan K, Griffiths SP, Cook N, Court R, Wigley DB. DNA binding to RecD: role of the 1B domain in SF1B helicase activity. EMBO J 2008;27(16):2222-9

Zhang X,Wigley DB. The 'glutamate switch' provides a link between ATPase activity and ligand binding in AAA+ proteins. Nat Struct Mol Biol 2008;15(11):1223-7s

2007

Court R, Cook N, Saikrishnan K, Wigley D. The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition. J Mol Biol 2007; 371(1):25-33

Gaudier M, Schuwirth BS, Westcott SL, Wigley DB. Structural basis of DNA replication origin recognition by an ORC protein. Science 2007; 317(5842):1213-1216

2006

Grainge I, Gaudier M, Schuwirth BS, Westcott SL, Sandall J, Atanassova N, Wigley DB. Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernix. J Mol Biol 2006; 363(2):355-369

Petalcorin MIR, Sandall J, Wigley DB, Boulton SJ. CeBRC-2 stimulates D-loop formation by RAD-51 and promotes DNA single-strand annealing. J Mol Biol 2006; 361(2):231-242

Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB. Communication between subunits within an archaeal clamp-loader complex. EMBO J 2006; 25(10):2209-2218

2005

Court R, Chapman L, Fairall L, Rhodes D. How the human telomeric proteins TRF1 and TRF2 recognizetelomeric DNA: a view from high-resolution crystal structures. EMBO Rep 2005; 6(1):39-45

2004

Seybert A, Wigley DB. Distinct roles for ATP binding and hydrolysis at individual subunits of an archaeal clamp loader. EMBO J 2004; 23(6):1360-1371

Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB. Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature 2004; 432(7014):187-193

Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB. Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix. J Mol Biol 2004; 343(3):547-557

2003

Grainge I, Scaife S, Wigley DB. Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus. Nucleic Acids Res 2003; 31(16):4888-4898

2002

Dillingham MS, Wigley DB, Webb MR. Direct measurement of single-stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine. Biochemistry 2002; 41(2):643-651

Noirot-Gros MF, Soultanas P, Wigley DB, Ehrlich SD, Noirot P, Petit M-A. The beta-propeller protein YxaL increases the processivity of the PcrA helicase. Mol Genet Genomics 2002; 267(3):391-400

Polard P, Marsin S, McGovern S, Velten M, Wigley DB, Ehrlich SD, Bruand C. Restart of DNA replication in Gram-positive bacteria: functional characterisation of the Bacillus subtilis PriA initiator. Nucleic Acids Res 2002; 30(7):1593-1605

Seybert A, Scott DJ, Scaife S, Singleton MR, Wigley DB. Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Nucleic Acids Res 2002; 30(20):4329-4338

Singleton MR, Wentzell LM, Liu YL, West SC, Wigley DB. Structure of the single-strand annealing domain of human RAD52 protein. Proc Natl Acad Sci U S A 2002; 99(21):13492-13497

Soultanas P, Wigley DB. Site-directed mutagenesis reveals roles for conserved amino acid residues in the hexameric DNA helicase DnaB from Bacillus stearothermophilus. Nucleic Acids Res 2002; 30(18):4051-4060

Other Publications

Singleton MR, Dillingham MS, Wigley DB. Structure and mechanism of helicases and nucleic acid translocases. Annu Rev Biochem 2007; 76:23-50

Wigley DB. RecBCD: The supercar of DNA repair. Cell 2007; 131(4):651-653

Takahashi TS, Wigley DB, Walter JC. Pumps, paradoxes and ploughshares: mechanism of the MCM2-7 DNA helicase. Trends Biochem Sci 2005; 30(8):437-444

Singleton MR, Wigley DB. Multiple roles for ATP hydrolysis in nucleic acid modifying enzymes. EMBO J 2003; 22(18):4579-4583

Singleton MR, Wigley DB. Modularity and specialization in superfamily 1 and 2 helicases. J Bacteriol 2002; 184(7):1819-1826