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Stem Cell analysis: Phenotyping
Although it is believed that the most primitive of stem cells may express few markers, combination strategies have been devised for the identification of stem cells in particular tissues. These include skin, breast and haematopoietic cells. In the haematopoietic system the most commonly used strategy is the so-called KTLS subset. This identifies cells that are: - c-kit positive
- Thy-1 dim
- Lineage negative and
- Sca-1 positive.
These cells have been shown to be enriched for long-term repopulating cells. Below is an example of the gating strategy we would use and again, back-gating of the KTLS population shows that these cells have scatter characteristics consistent with a stem cell population.
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