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Head of Laboratory: Sharon A. Tooze
The goal of the Secretory Pathways Laboratory is to increase our understanding of organelle biogenesis, a process essential for cell function and survival. Westudy the formation of two specialized intracellular organelles: secretory granules, a model for organelle formation from the Golgi complex, and autophagosomes, organellesthat form de novo in the cytosol during nutrient depravation conditions. Our studies of how secretory granules are formed in neuroendocrine cells focus on two fundamental steps: homotypic membrane fusion and vesicle formation. Our studies on autophagy aim to understand the initiation, formation, and maturation of autophagosomes at the molecular level.
Keywords: regulated secretion, immature secretory granule, autophagy
Laboratory Members
SPL lab members, from left to right: Ed Chan, Or Kakhlon, Malika Ahras, Andy Young, Wen Hu, Robert Koechl, John Copier, Harold Jefferies, Grant Otto
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The main areas of interest in the lab are diagrammed. Right, the regulated secretory pathway. Immature secretory granules (ISGs) originate from the trans-Golgi network (TGN), and undergo homotypic fusion and clathrin-coat remodelling via clathrin coated vesicles (CCVs), becoming mature secretory granules (MSGs). Left, the autophagic pathway. Phagophores, or pre-autophagosomal structures (PAS) enclose cytosol and organelles becoming immature autophagic vacuoles (AV). AVs fuse with endosomes (not shown) and become degradative autophagic vacuoles (AVds).
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